Affiliations 1 Programa de Pós-Graduação Em Farmácia (PPGFar), Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.; 2 Departamento de Análises Clínicas-CCS, Universidade Federal de Santa Catarina, Campus Universitário-Trindade, Florianópolis, SC, 88040-970, Brazil.Background: Diabetic nephropathy (DN) is one of the leading causes of chronic kidney disease (CKD) worldwide, tubular injury is the driving force during the pathogenesis and progression of DN. Thus, we aim to utilize the connectivity map (CMap) with renal tubulointerstitial transcriptomic profiles of biopsy-proven DN to identify novel drugs for treating DN.Methods: We interrogated the CMap ...NLRP3 inflammasome is a crucial regulator of pro-inflammatory cytokines (IL-1β and IL-18) production through activating caspase-1. Non-coding RNAs (ncRNAs) are a class of RNA transcripts lacking the ability to encode peptides or proteins. Its dysregulation leads to the development and progression of inflammation in diseases.Animal Care and Surgery. All practices on mice in this study were performed in compliance with the institutional guidelines on the scientific use of living animals at Interdisciplinary Research Center on Biology and Chemistry, the Chinese Academy of Sciences, as well as “Principles of Laboratory Animal Care” by National Institute of Health, United States (No. 86-23).Aug 1, 2023 · The interactive shell stores your history which can be accessed using the up and down keys. The history is saved in the ~/.php_history file. The CLI SAPI provides the php.ini settings cli.pager and cli.prompt. The cli.pager setting allows an external program (such as less) to act as a pager for the output instead of being displayed directly on ... PHP is a server scripting language, and a powerful tool for making dynamic and interactive Web pages. PHP is a widely-used, free, and efficient alternative to competitors such as Microsoft's ASP. Start learning PHP now ».PHP is a server scripting language, and a powerful tool for making dynamic and interactive Web pages. PHP is a widely-used, free, and efficient alternative to competitors such as Microsoft's ASP. Start learning PHP now ».Example Get your own PHP Server. When the user fills out the form above and clicks the submit button, the form data is sent for processing to a PHP file named "welcome.php". The form data is sent with the HTTP POST method. To display the submitted data you could simply echo all the variables. The "welcome.php" looks like this:PHP Operators. Operators are used to perform operations on variables and values. PHP divides the operators in the following groups: Arithmetic operators. Assignment operators. Comparison operators. Increment/Decrement operators. Logical operators. String operators.30-2005 ™. Iscove's Modified Dulbecco's Medium (IMDM) contains 4 mM L-glutamine, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate. It has applications for cell culture, cell growth, and viability. Product category. Media.Figure 2 . Cell 2019 177384-398.e11DOI: (10.1016/j.cell.2019.01.039) Copyright © 2019 Elsevier Inc. Terms and Conditions. TBK1 Is a Component of the MyddosomeNerve injury induces profound and complex changes at molecular and cellular levels, leading to axonal self-destruction as well as immune and inflammatory responses that may further promote neurodegeneration. To better understand how neural injury changes the proteome within the injured nerve, we set up a mouse model of sciatic nerve injury (SNI) and conducted an unbiased, quantitative ...We refer to the antiinflammatory function of IRF3 as the repression of IRF3-mediated NF-κB activity (RIKA). RIKA, in addition to virus infection, also inhibits inflammatory gene expression by nonviral stimuli. Furthermore, we show RIKA contributes to the antiviral functions of IRF3 in cells and mice.Instagram Mar 15, 2022 · There are two main ways to install PHP on a Windows®-based computer: download the Windows Installer or use the Windows Zip file from the PHP Web site. Either method will get PHP working, but both have some extra steps that are needed to make PHP work well. Windows Installer The user friendly PHP online compiler that allows you to Write PHP code and run it online. The PHP text editor also supports taking input from the user and standard libraries.PHP Operators. Operators are used to perform operations on variables and values. PHP divides the operators in the following groups: Arithmetic operators. Assignment operators. Comparison operators. Increment/Decrement operators. Logical operators. String operators. BEI Resources macrophages Macrophages, supplied by BEI Resources, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Nanoscale PAPER Cite this: Nanoscale, 2022, 14, 4935 Received 19th October 2021, Accepted 7th February 2022 DOI: 10.1039/d1nr06922e rsc.li/nanoscalePractice is key to mastering coding, and the best way to put your PHP knowledge into practice is by getting practical with code. Use W3Schools Spaces to build, test and deploy code. The code editor lets you write and practice different types of computer languages. It includes PHP, but you can use it for other languages too.NOD-like receptor (NLR) family pyrin domain-containing-3 (NLRP3) inflammasome is implicated in inflammation-associated diseases such as multiple sclerosis, Parkinson's disease, and stroke. Targeting the NLRP3 inflammasome is beneficial to these diseases, but few NLRP3 inflammasome-selective inhibito …Salmonella typhimurium (S. typhimurium) infection of macrophage induces NLRC4 inflammasome-mediated production of the pro-inflammatory cytokines IL-1β.Post-translational modifications on NLRC4 are critical for its activation.Factors to Consider When Choosing a PHP Framework. Best PHP Frameworks in 2023 (Comparison Table) 10 Best PHP Frameworks in 2023 (Ranked) Laravel. Symfony. Yii 2. CakePHP. CodeIgniter. Zend Framework.Affiliations 1 Programa de Pós-Graduação Em Farmácia (PPGFar), Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.; 2 Departamento de Análises Clínicas-CCS, Universidade Federal de Santa Catarina, Campus Universitário-Trindade, Florianópolis, SC, 88040-970, Brazil. BEI Resources macrophages Macrophages, supplied by BEI Resources, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and moreInstagram As the seminal step in gluconeogenesis, pyruvate carboxylase (PCB) plays a dominant role in controlling gluconeogenesis. 3 Although the correlation between tumor prosperity and the canonical ...A comment in PHP code is a line that is not executed as a part of the program. Its only purpose is to be read by someone who is looking at the code. Comments can be used to: Let others understand your code. Remind yourself of what you did - Most programmers have experienced coming back to their own work a year or two later and having to re ...IMMUNOLOGY ESCRT-dependent membrane repair negatively regulates pyroptosis downstream of GSDMD activation Sebastian Rühl1,2, Kateryna Shkarina 3*, Benjamin Demarco3*, Rosalie Heilig *,MySQL is a database system used on the web. MySQL is a database system that runs on a server. MySQL is ideal for both small and large applications. MySQL is very fast, reliable, and easy to use. MySQL uses standard SQL. MySQL compiles on a number of platforms. MySQL is free to download and use. MySQL is developed, distributed, and supported by ...PHP 8.3.0 RC 1 available for testing. Predefined Variables. Predefined Exceptions. Predefined Interfaces and Classes. Predefined Attributes. Supported Protocols and Wrappers. Variable and Type Related Extensions. "MyClass::myFunc () ""OtherClass::myFunc () ". A class constant, class property (static), and class function (static) can all share ...Download scientific diagram | Generation of monoclonal iBMDM cells by single-cell cloning. iBMDMs at a concentration of 1 × 10 6 cell per mL are diluted 1:1000 and then 1:100 to obtain a ... pub 2048R/31CBD89E 2016-12-08 Key fingerprint = 5289 95BF EDFB A719 1D46 839E F9BA 0ADA 31CB D89E uid Joe Watkins <[email protected]> pub rsa4096 2021-04-26 [SC] [expires: 2025-11-24] 39B6 4134 3D8C 104B 2B14 6DC3 F9C3 9DC0 B969 8544 uid [ultimate] Ben Ramsey <[email protected]> sub rsa4096 2021-04-26 [E] [expires: 2025-11-24] pub rsa4096 2021-04-01 [SC] F1F6 9223 8FBC 1666 E5A5 CCD4 199F 9DFE F6FF ...Sep 16, 2015 · Inflammatory caspases (caspase-1, -4, -5 and -11) are critical for innate defences. Caspase-1 is activated by ligands of various canonical inflammasomes, and caspase-4, -5 and -11 directly ... Enter your user name and password, then click Log in. User name: Help Password: Log in 邵峰组重磅牛文：神秘蛋白 GSDMD如何“玩转”细胞焦亡. 小编在前期介绍了炎性caspases-1介导的细胞焦亡，这期小编将要深入介绍炎性caspases介导的细胞焦亡的关键底物蛋白GSDMD。. 该底物蛋白是细胞发生焦亡的充要条件。. 该文章是邵峰课题组发表在Nature上的大作 ...邵峰组重磅牛文：神秘蛋白 GSDMD如何“玩转”细胞焦亡. 小编在前期介绍了炎性caspases-1介导的细胞焦亡，这期小编将要深入介绍炎性caspases介导的细胞焦亡的关键底物蛋白GSDMD。. 该底物蛋白是细胞发生焦亡的充要条件。. 该文章是邵峰课题组发表在Nature上的大作 ...The endoplasmic reticulum (ER) is the largest organelle in the cell and plays numerous functions vital for maintaining cellular homeostasis. It is the primary site for protein synthesis of both secreted and integral membrane proteins and the export of newly synthesized proteins to other cellular organelles.Jan 24, 2016 · The advent of deep sequencing technologies has provided us with an unprecedented view of the human genome. Over 85 % of the genome is actively transcribed, yet we do not know the function of the vast majority of these RNA transcripts. Long noncoding RNAs (lncRNA)... Figure Legend Snippet: Bullatine A inhibited the expression of inflammatory genes in LPS-activated microglia and macrophages. The mRNA levels of IL-1β (A), IL-6 (B), iNOS (C) and TNF-α (D) in BV-2 cells treated with LPS (1 µg/mL) and BA (10–80 μM) for 6 h were detected by quantitative RT-PCR.Figure Legend Snippet: Bullatine A inhibited the expression of inflammatory genes in LPS-activated microglia and macrophages. The mRNA levels of IL-1β (A), IL-6 (B), iNOS (C) and TNF-α (D) in BV-2 cells treated with LPS (1 µg/mL) and BA (10–80 μM) for 6 h were detected by quantitative RT-PCR.pub 2048R/31CBD89E 2016-12-08 Key fingerprint = 5289 95BF EDFB A719 1D46 839E F9BA 0ADA 31CB D89E uid Joe Watkins <[email protected]> pub rsa4096 2021-04-26 [SC] [expires: 2025-11-24] 39B6 4134 3D8C 104B 2B14 6DC3 F9C3 9DC0 B969 8544 uid [ultimate] Ben Ramsey <[email protected]> sub rsa4096 2021-04-26 [E] [expires: 2025-11-24] pub rsa4096 2021-04-01 [SC] F1F6 9223 8FBC 1666 E5A5 CCD4 199F 9DFE F6FF ... Nanoscale PAPER Cite this: Nanoscale, 2022, 14, 4935 Received 19th October 2021, Accepted 7th February 2022 DOI: 10.1039/d1nr06922e rsc.li/nanoscale Nov 25, 2020 · Activation of TLR7 by small imidazoquinoline molecules such as R848 or R837 initiates signaling cascades leading to the activation of transcription factors, such as AP-1, NF-κB, and interferon regulatory factors (IRFs) and afterward to the induction of cytokines and anti-viral Type I IFNs. In general, TLRs mediate these effects by utilizing different intracellular signaling molecules, one of ... Nanoscale PAPER Cite this: Nanoscale, 2022, 14, 4935 Received 19th October 2021, Accepted 7th February 2022 DOI: 10.1039/d1nr06922e rsc.li/nanoscaleNerve injury induces profound and complex changes at molecular and cellular levels, leading to axonal self-destruction as well as immune and inflammatory responses that may further promote neurodegeneration. To better understand how neural injury changes the proteome within the injured nerve, we set up a mouse model of sciatic nerve injury (SNI) and conducted an unbiased, quantitative ... 37 The innate immune system is complex and involves a huge number of proteins. Hence, studies into mechanisms of innate immune activation often rely heavily on access to a wide array ofFigure Legend Snippet: Bullatine A inhibited the expression of inflammatory genes in LPS-activated microglia and macrophages. The mRNA levels of IL-1β (A), IL-6 (B), iNOS (C) and TNF-α (D) in BV-2 cells treated with LPS (1 µg/mL) and BA (10–80 μM) for 6 h were detected by quantitative RT-PCR.Factors to Consider When Choosing a PHP Framework. Best PHP Frameworks in 2023 (Comparison Table) 10 Best PHP Frameworks in 2023 (Ranked) Laravel. Symfony. Yii 2. CakePHP. CodeIgniter. Zend Framework.Practice is key to mastering coding, and the best way to put your PHP knowledge into practice is by getting practical with code. Use W3Schools Spaces to build, test and deploy code. The code editor lets you write and practice different types of computer languages. It includes PHP, but you can use it for other languages too. Enter your user name and password, then click Log in. User name: Help Password: Log in Here, we describe a robust protocol for the generation of immortalized bone marrow-derived macrophages (iBMDMs) from primary murine bone marrow cells. We further provide general protocols for ...Figure Legend Snippet: Bullatine A inhibited the expression of inflammatory genes in LPS-activated microglia and macrophages. The mRNA levels of IL-1β (A), IL-6 (B), iNOS (C) and TNF-α (D) in BV-2 cells treated with LPS (1 µg/mL) and BA (10–80 μM) for 6 h were detected by quantitative RT-PCR.Corresponding Author. Xiang Gao [email protected] MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing University, Nanjing, ChinaEnter your user name and password, then click Log in. User name: Help Password: Log in Aug 14, 2023 · Our basic PHP website is going to feature a home page, including biographical information and some images. For this simple PHP website, you're going to create a single PHP page populated by content from three HTML pages. The index.php file you create can then be edited by adjusting the words and images from the original HTML files. Step 1- Create a HTML PHP Login Form. Step 2: Create a CSS Code for Website Design. View More. In today's digital age, the importance of online security cannot be ignored. As a result, creating a login form is an essential feature for any website that requires user authentication. The following is a guide on creating a login form in PHP.Aug 18, 2023 · Step 1- Create a HTML PHP Login Form. Step 2: Create a CSS Code for Website Design. View More. In today's digital age, the importance of online security cannot be ignored. As a result, creating a login form is an essential feature for any website that requires user authentication. The following is a guide on creating a login form in PHP. Sep 16, 2015 · Inflammatory caspases (caspase-1, -4, -5 and -11) are critical for innate defences. Caspase-1 is activated by ligands of various canonical inflammasomes, and caspase-4, -5 and -11 directly ... Feb 2, 2022 · Next, we investigated whether intervening PCB in TAMs could influence tumor progression and anti-tumor immunity. As PCB suppression could be reversed by alleviating hypoxia (Fig. 1d), we treated ... Aug 1, 2023 · PHP (recursive acronym for PHP: Hypertext Preprocessor) is a widely-used open source general-purpose scripting language that is especially suited for web development and can be embedded into HTML. Magupalli et al. report that for NLRP3- and pyrin-mediated inflammasomes, their assembly and downstream functions occur at the microtubule-organizing center (MTOC). This process requires the dynein adaptor HDAC6, which is also a central player in aggresome formation and autophagosomal degradation at the MTOC.Aug 1, 2023 · Here's how to run dual PHP instances with PHP 5.2 and any previous PHP on Windows 2003: 1. Right-click My Computer, go to Advanced tab, and click on Environment Variables. Add the two installations and their EXT directories to the Path variable. For example, add: c:\php;c:\php\ext;c:\TMAS\php;c:\tmas\php\ext; Feb 15, 2021 · Salmonella typhimurium (S. typhimurium) infection of macrophage induces NLRC4 inflammasome-mediated production of the pro-inflammatory cytokines IL-1β.Post-translational modifications on NLRC4 are critical for its activation. Author summary Our immune system protects us from infections caused by bacteria, viruses, fungi, and other organisms that we encounter regularly. The majority of these organisms have not evolved to cause us harm and are either tolerated within us or else are removed by the immune system. However, when the immune system is compromised, whether through illness, medication, or genetic ...Figure Legend Snippet: Bullatine A inhibited the expression of inflammatory genes in LPS-activated microglia and macrophages. The mRNA levels of IL-1β (A), IL-6 (B), iNOS (C) and TNF-α (D) in BV-2 cells treated with LPS (1 µg/mL) and BA (10–80 μM) for 6 h were detected by quantitative RT-PCR.mysqli::query — Performs a query on the database. mysqli::real_connect — Opens a connection to a mysql server. mysqli::real_escape_string — Escapes special characters in a string for use in an SQL statement, taking into account the current charset of the connection. mysqli::real_query — Execute an SQL query.Aug 1, 2023 · The interactive shell stores your history which can be accessed using the up and down keys. The history is saved in the ~/.php_history file. The CLI SAPI provides the php.ini settings cli.pager and cli.prompt. The cli.pager setting allows an external program (such as less) to act as a pager for the output instead of being displayed directly on ... PHP-GTK related documentation is hosted on the PHP-GTK website. Documentation of PEAR and the various packages can be found on a separate server. You can still read a copy of the original PHP/FI 2.0 Manual on our site, which we only host for historical purposes. The same applies to the PHP 3 Manual .This function of Peli1 is specific for TIR-domain-containing adapter-inducing interferon-β (TRIF)-dependent TLRs, while Peli1 is dispensable for NF-κB activation by the MyD88-dependent TLRs and IL-1 receptor (IL-1R). In the present study, we demonstrate that Peli1 has a crucial role in NLRP3 inflammasome activation.Nanoscale PAPER Cite this: Nanoscale, 2022, 14, 4935 Received 19th October 2021, Accepted 7th February 2022 DOI: 10.1039/d1nr06922e rsc.li/nanoscale 1. I had the same problem and i fixed some steps : open the command line in administrator right (windows + r + type 'cmd') write the PATH where you're your dll file: C:\Windows\system32\regsvr32 xwizards.dll (it's example) a window show up with "DLLRegisterServer success".Nanoscale PAPER Cite this: Nanoscale, 2022, 14, 4935 Received 19th October 2021, Accepted 7th February 2022 DOI: 10.1039/d1nr06922e rsc.li/nanoscaleBackground: Diabetic nephropathy (DN) is one of the leading causes of chronic kidney disease (CKD) worldwide, tubular injury is the driving force during the pathogenesis and progression of DN. Thus, we aim to utilize the connectivity map (CMap) with renal tubulointerstitial transcriptomic profiles of biopsy-proven DN to identify novel drugs for treating DN.Methods: We interrogated the CMap ...Apr 4, 2019 · The signaling organelles of the innate immune system consist of oligomeric protein complexes known as supramolecular organizing centers (SMOCs). Examples of SMOCs include myddosomes and inflammasomes, which respectively induce transcription-dependent and -independent inflammatory responses. The common use of oligomeric structures as signaling ... PHP 8.3.0 RC 1 available for testing. Predefined Variables. Predefined Exceptions. Predefined Interfaces and Classes. Predefined Attributes. Supported Protocols and Wrappers. Variable and Type Related Extensions. "MyClass::myFunc () ""OtherClass::myFunc () ". A class constant, class property (static), and class function (static) can all share ...Dec 1, 2019 · PHP is a server-side scripting language created in 1995 by Rasmus Lerdorf. PHP is a widely-used open source general-purpose scripting language that is especially suited for web development and can be embedded into HTML. What is PHP used for? As of October 2018, PHP is used on 80% of websites whose server-side language is known. It is typically ... Apr 4, 2019 · The signaling organelles of the innate immune system consist of oligomeric protein complexes known as supramolecular organizing centers (SMOCs). Examples of SMOCs include myddosomes and inflammasomes, which respectively induce transcription-dependent and -independent inflammatory responses. The common use of oligomeric structures as signaling ... Here's how to run dual PHP instances with PHP 5.2 and any previous PHP on Windows 2003: 1. Right-click My Computer, go to Advanced tab, and click on Environment Variables. Add the two installations and their EXT directories to the Path variable. For example, add: c:\php;c:\php\ext;c:\TMAS\php;c:\tmas\php\ext;Jul 24, 2017 · MyD88 adaptor-like (MAL) is a critical protein in innate immunity, involved in signaling by several Toll-like receptors (TLRs), key pattern recognition receptors (PRRs). PHP Operators. Operators are used to perform operations on variables and values. PHP divides the operators in the following groups: Arithmetic operators. Assignment operators. Comparison operators. Increment/Decrement operators. Logical operators. String operators.
pub 2048R/31CBD89E 2016-12-08 Key fingerprint = 5289 95BF EDFB A719 1D46 839E F9BA 0ADA 31CB D89E uid Joe Watkins <[email protected]> pub rsa4096 2021-04-26 [SC] [expires: 2025-11-24] 39B6 4134 3D8C 104B 2B14 6DC3 F9C3 9DC0 B969 8544 uid [ultimate] Ben Ramsey <[email protected]> sub rsa4096 2021-04-26 [E] [expires: 2025-11-24] pub rsa4096 2021-04-01 [SC] F1F6 9223 8FBC 1666 E5A5 CCD4 199F 9DFE F6FF ... . Karlo libby funeral home obituaries
SignificanceToll-like receptor (TLR) signaling pathways are targeted to limit inflammation in immune cells. TLRs use adaptor proteins to drive inflammatory signaling platforms for effective microbi...邵峰组重磅牛文：神秘蛋白 GSDMD如何“玩转”细胞焦亡. 小编在前期介绍了炎性caspases-1介导的细胞焦亡，这期小编将要深入介绍炎性caspases介导的细胞焦亡的关键底物蛋白GSDMD。. 该底物蛋白是细胞发生焦亡的充要条件。. 该文章是邵峰课题组发表在Nature上的大作 ... The user friendly PHP online compiler that allows you to Write PHP code and run it online. The PHP text editor also supports taking input from the user and standard libraries.PHP is a server-side scripting language designed specifically for web development. It is open-source which means it is free to download and use. It is very simple to learn and use. The files have the extension “.php”. Rasmus Lerdorf inspired the first version of PHP and participated in the later versions. It is an interpreted language and ...30-2005 ™. Iscove's Modified Dulbecco's Medium (IMDM) contains 4 mM L-glutamine, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate. It has applications for cell culture, cell growth, and viability. Product category. Media.Figure 2 . Cell 2019 177384-398.e11DOI: (10.1016/j.cell.2019.01.039) Copyright © 2019 Elsevier Inc. Terms and Conditions. TBK1 Is a Component of the MyddosomeJan 20, 2023 · Mammalian cells are equipped with pattern recognition receptors (PRRs) that detect molecular evidence of infection or tissue injury. Among these PRRs is the enzyme cyclic guanosine monophosphate–adenosine monophosphate (GMP–AMP) synthase (cGAS), which synthesizes the second messenger 2′3′-cyclic GMP-AMP (cGAMP) upon binding to double-stranded DNA (dsDNA) in the cytosol (1, 2). Here, we show site-specific caspase-4/11 autoprocessing, generating a p10 product, is required and sufficient for cleaving GSDMD and inducing pyroptosis. The p10-form autoprocessed caspase-4/11 binds the GSDMD-C domain with a high affinity. Structural comparison of autoprocessed and unprocessed capase-11 identifies a β sheet induced by the ...Nanoscale PAPER Cite this: Nanoscale, 2022, 14, 4935 Received 19th October 2021, Accepted 7th February 2022 DOI: 10.1039/d1nr06922e rsc.li/nanoscale Jul 14, 2022 · Background: Diabetic nephropathy (DN) is one of the leading causes of chronic kidney disease (CKD) worldwide, tubular injury is the driving force during the pathogenesis and progression of DN. Thus, we aim to utilize the connectivity map (CMap) with renal tubulointerstitial transcriptomic profiles of biopsy-proven DN to identify novel drugs for treating DN.Methods: We interrogated the CMap ... Enter your user name and password, then click Log in. User name: Help Password: Log inWe treated WT and Mal −/− iBMDMs with the protein synthesis inhibitor-cycloheximide and studied its effect on R848-induced expression of Ifnβ and Irf7 mRNA ( Figure 4 A,B). Cycloheximide ... Here, we describe a robust protocol for the generation of immortalized bone marrow-derived macrophages (iBMDMs) from primary murine bone marrow cells. We further provide general protocols for ...Affiliations 1 Programa de Pós-Graduação Em Farmácia (PPGFar), Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.; 2 Departamento de Análises Clínicas-CCS, Universidade Federal de Santa Catarina, Campus Universitário-Trindade, Florianópolis, SC, 88040-970, Brazil..